DOI: 10.31220/agriRxiv.2024.00249

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  Abstract: Abstract The threat of global warming to plant survival, along with its adverse effects on growth and agricultural productivity, underscores the urgency of developing thermotolerant crops. Achieving this goal necessitates a thorough comprehension of plant responses to heat stress at the molecular level. To address this, we conducted an investigation into proteome dynamics in Arabidopsis thaliana seedlings exposed to moderate heat stress (30°C). Employing a novel approach combining 15N-stable isotope labeling and the ProteinTurnover algorithm facilitated a detailed examination of proteomic changes across various cellular fractions. Our study unveiled significant alterations in the turnover rates of 571 proteins, with a median increase of 1.4-fold, indicative of heightened protein dynamics in response to heat stress. Notably, soluble proteins in the roots exhibited comparatively minor changes, suggesting tissue-specific adaptive mechanisms. The analysis also revealed substantial turnover variations in proteins associated with redox signaling, stress response, and metabolism, underscoring the intricate nature of the response network. Conversely, proteins involved in carbohydrate metabolism and mitochondrial ATP synthesis demonstrated minimal turnover fluctuations, highlighting their inherent stability. This comprehensive assessment sheds light on the proteomic adaptations of Arabidopsis seedlings to moderate heat stress, elucidating the delicate balance between proteome stability and adaptability. These insights contribute to our understanding of plant thermal resilience and offer valuable support for the development of crops endowed with enhanced thermotolerance.

  Key words: proteins; crops; arabidopsis seedlings; the response; turnover; moderate heat stres

  Submit time: 2 May 2024

  Copyright: The copyright holder for this preprint is the author/funder, who has granted agriXiv a license to display the preprint in perpetuity.
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