DOI：10.12343/Agrixiv.202606.00001  CSTR：31252.36.agrixiv.202606.00001

Telomere-to-Telomere Genome Assembly and Its Application Progress in Domesticated Animal Genetics Research

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  Abstract: The primary determinant of genome assembly difficulty is not genome size per se, but rather the repetitive architecture of the genome. Repetitive regions associated with specialized chromosomal structures are substantially longer than the reads generated by short-read sequencing technologies, thereby posing considerable challenges to accurate assembly. Long-read sequencing technologies, however, are capable of producing reads that exceed the length of repetitive sequences, making it possible to construct seamless telomere-to-telomere (T2T) assemblies and thus providing novel strategies for elucidating genome structure and function. T2T assembly is more readily achievable in animals than in polyploid plant genomes, and notable successes have been achieved across a range of domesticated animal species, with the field now entering a phase of rapid expansion. This review describes the methodological foundations of T2T genome assembly and surveys the current research progress of T2T genome research in domesticated animals. It further examines the applications of T2T genomes in domesticated animal genetics research and discusses the associated challenges, with the aim of advancing the use of complete genome assemblies in the study of species evolution, genetic improvement, and related disciplines.

  Key words: Domesticated animal; T2T assembly; assembly methods; applications; challenges

  提交时间：2026-06-08

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• 序号	提交日期	编号	操作
  1	2026-06-08	10.12343/Agrixiv.202606.00001V1	下载

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